macvector steps to compare two sequences

The mechanism by which pediocin AcH kills bacterial cells has been extensively investigated. This peptide is highly active against food pathogens such as Listeria monocytogenes and Clostridium botulinum which cause many deaths worldwide each year ( Jack et al. Pediocin AcH (same as pediocin PA-1) is a 44-amino-acid class IIa bacteriocin produced primarily by strains of the genus Pediococcus ( Ennahar et al. These plasmids should be useful for expressing pediocin AcH in Pediococcus and Lactobacillus strains used in food production. Significance and Impact of the Study: This is the first demonstration that the expression of a class IIa bacteriocin is directed by a common gene cassette that has been disseminated to unique plasmids in different genera of lactic acid bacteria.

All four genes needed for production are present within a single plasmid in each strain. The amino acid sequences of the encoded proteins are highly conserved between plasmids.Ĭonclusions: Pediocin AcH production genes are located within a plasmid-borne operon cassette in all lactic acid bacterial strains examined to date. Structure, immunity and secretion system genes are linked together in the operons, and the promoter sequences are the same. Methods and Results: Plasmid curing, Southern blot hybridization, and DNA sequence analysis indicate that pediocin AcH production genes are encoded by highly similar operons in unique plasmids designated pATO77 from P. Aims: To determine the locations and sequences of pediocin AcH production genes in Pediococcus parvulus ATO77 from vegetables, Lactobacillus plantarum WHE92 from Muenster cheese, and a lactose-fermenting isolate Pediococcus pentosaceus S34 from buffalo milk.

Macvector steps to compare two sequences